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After removing these exclusions, 64 men aged 80 –89 remained, but only five were apparently healthy. Because we report reference ranges for apparently healthy men only, we removed the 80- to 89-yearolds due to scarce data. The resulting subsamples were 1677, 1031 and 631 for T1, T2 and T3, respectively, for a grand total of 3339 observations. Descriptive analyses revealed that men who had chronic disease, were on medication, or who had high BMI tended to have lower total, free and bio-available T levels, whereas smokers tended to have higher levels, steroid effects use of anabolic, steroid use anabolic effects of. To prevent the effect of smoking from canceling out the effects of the other components, the apparently healthy variable was split into two variables for modelling purposes: (a) met all of conditions 1– 4 of the definition and (b) nonsmoker. We chose to define reference ranges using percentiles rather than the commonly used formula, mean ± 2SD. _ERRROR_

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The humoral factors that have been identified as muscle growth factors are fibroblast growth factor (FGF), the insulin-like growth factors (IGF-I and -II), and transforming growth factor-beta (TGF- ) [1 ]. Activation of the PI3 kinase pathway can induce skeletal muscle hypertrophy, defined as an increase in skeletal muscle mass. In mammals, skeletal muscle hypertrophy results from an increase in the sizes of preexisting skeletal muscle fibers rather than in the number of fibers. The effects of the PI3 kinase pathway on skeletal muscle have been detected most prominently downstream of IGF-I signaling [2 ]. Recently, mechanical stimuli have been implicated in the regulation of skeletal muscle mass and the maintenance of muscle mass [3.


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